ABSTRACT
BACKGROUND & OBJECTIVE: The reason for lack of data on burden of Haemophilus influenzae type b (Hib) in developing countries was mainly failure of detection of this fastidious organism in laboratories. Use of isovitalex (IVX) was suggested as an essential supplement for growing this organism. This study was carried out to investigate the impact of IVX supplementation to chocolate agar for detection of Hib. METHODS: Chocolate agar with and without supplementation of IVX was prepared. Clinical samples as well as reference strains of Hib were simultaneously cultured on both the media. RESULTS: H. influenzae isolates (N=194) were simultaneously grown on chocolate agar (CA) with and without isovitalex (IVX). Average colony size of H. influenzae on CA with IVX (CA-IVX) was larger only by 0.10 cm (range 0.05 to 0.16 cm) compared to CA alone. Addition of IVX to CA increased the cost of media by 2.1-fold. INTERPRETATION & CONCLUSION: Isovitalex is not essential for the isolation and growth of H. influenzae almost halving the cost.
Subject(s)
Cell Culture Techniques/methods , Culture Media/chemistry , Growth Substances/chemistry , Haemophilus influenzae/growth & development , Haemophilus influenzae/isolation & purificationABSTRACT
Mouse spleen cells activated in a mixed lymphocyte reaction release a soluble factor, which induces a significant proliferative response in fresh mouse spleen cells. This proliferation inducing factor (PIF) was found to be heat stable (90 degrees C for 45 min) and also resistant to trypsin or chymotrypsin treatment. By using a sizing HPLC column, the molecular weight of PIF appears to be 25 kDa. Mouse spleen cells treated with anti-thy-1 + complement lost Con-A induced proliferative responses but responded well to PIF. B cell depleted spleen cells obtained by negative selection panning, did not respond to PIF. These results indicate that B cells proliferated in response to PIF. Polymixin-B, which blocks the B cell proliferative response to LPS, did not inhibit PIF induced proliferation.
Subject(s)
Mice , Animals , B-Lymphocytes/physiology , B-Lymphocytes/drug effects , Bone Marrow/metabolism , Cell Division/physiology , Chromatography, High Pressure Liquid , Chymotrypsin/pharmacology , Dose-Response Relationship, Drug , Growth Substances/pharmacology , Growth Substances/chemistry , Hot Temperature , Mice, Inbred BALB C , Mice, Inbred C57BL , Molecular Weight , Polymyxin B/pharmacology , Protein Denaturation , Spleen/metabolism , Thymus Gland/metabolism , Trypsin/pharmacologyABSTRACT
There are now several hormone and hormone-like agents that can improve the rate of growth and efficiency of feed use of farm animals. Feeding chickens with oral contraceptive steroids at the dose used by some poultry growers in Egypt has led to the formation of high estrogen residues in the muscles and the liver compared with controls. Electron microscopy of the livers of treated chickens revealed changes at the ultrastructural level. These alterations involve both the nucleus and cytoplasmic organelles, and are similar to those induced by 7, 12 dimethylbenz [a] anthracene in chickens. It is concluded that the use of oral contraceptive steroids in chickens as anabolic agent is a specific risk to chickens and probably to the consumer